A population genetics study of Anopheles darlingi (Diptera: Culicidae) from Colombia based on random amplified polymorphic DNA-polymerase chain reaction and amplified fragment lenght polymorphism markers

Memórias do Instituto Oswaldo Cruz

Ranulfo González¹  Richard Wilkerson²  Marco Fidel Suárez¹  Felipe García¹  Gerardo Gallego²  Heiber Cárdenas²  Carmen Elisa Posso¹  Myriam Cristina Duque² 

[1] ,Universidad del Valle Facultad de Ciencias y Facultad de Salud Cali,Colombia

关键词: amplified fragment length polymorphism;    Anopheles darlingi;    genetic diversity;    malaria vectors;    random amplified polymorphic DNA;    polymerase chain reaction;    Colombia;   

DOI  :  10.1590/S0074-02762007005000037

来源: SciELO

【 摘 要 】

The genetic variation and population structure of three populations of Anopheles darlingi from Colombia were studied using random amplified polymorphic markers (RAPDs) and amplified fragment length polymorphism markers (AFLPs). Six RAPD primers produced 46 polymorphic fragments, while two AFLP primer combinations produced 197 polymorphic fragments from 71 DNA samples. Both of the evaluated genetic markers showed the presence of gene flow, suggesting that Colombian An. darlingi populations are in panmixia. Average genetic diversity, estimated from observed heterozygosity, was 0.374 (RAPD) and 0.309 (AFLP). RAPD and AFLP markers showed little evidence of geographic separation between eastern and western populations; however, the F ST values showed high gene flow between the two western populations (RAPD: F ST = 0.029; Nm: 8.5; AFLP: F ST = 0.051; Nm: 4.7). According to molecular variance analysis (AMOVA), the genetic distance between populations was significant (RAPD:phiST = 0.084; AFLP:phiST = 0.229, P < 0.001). The F ST distances and AMOVAs using AFLP loci support the differentiation of the Guyana biogeographic province population from those of the Chocó-Magdalena. In this last region, Chocó and Córdoba populations showed the highest genetic flow.

【 授权许可】

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