Trypanosoma rangeli sialidase: lack of activity in stocks from Panama and other regions

Memórias do Instituto Oswaldo Cruz

Octavio E Sousa¹  Maritza Lombardo¹  Azael Saldaña¹ 

[1] ,University of Panama Faculty of Medicine Center for Research and Diagnosis of Parasitic Diseases,Republic of Panama

关键词: Trypanosoma rangeli;    sialidase;    heterogeneity;   

DOI  :  10.1590/S0074-02762005000300004

来源: SciELO

【 摘 要 】

A total of 33 crude and cloned Trypanosoma rangeli stocks found as natural infections in human from Panama and other endemic areas of Central and South America were evaluated as producers of sialidase (SA) activity through the MU-NANA fluorescence test. Negative results were observed in 6 of the isolates: Panama (4), Honduras (1), and Brazil (1). In addition, an immunoblotting analysis confirm the presence of the SA antigen in these stocks without enzymatic activity. These findings must be considered in the interpretation of the biological significance of T. rangeli SA and in the proper characterization and identification of this parasite.

【 授权许可】

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