Analysis of ancient DNA from coprolites: a perspective with random amplified polymorphic DNA-polymerase chain reaction approach

Memórias do Instituto Oswaldo Cruz

Alena M Iñiguez¹  Adauto Araújo²  Luiz Fernando Ferreira²  Ana Carolina P Vicente¹ 

[1] ,Fiocruz Instituto Oswaldo Cruz Departamento de GenéticaRio de Janeiro RJ ,Brasil

关键词: molecular paleoparasitology;    coprolites;    random amplified polymorphic DNA;    ancient DNA;    polymerase chain reaction;   

DOI  :  10.1590/S0074-02762003000900012

来源: SciELO

【 摘 要 】

The aim of this work was to determine approaches that would improve the quality of ancient DNA (aDNA) present in coprolites to enhance the possibility of success in retrieving specific sequence targets. We worked with coprolites from South American archaeological sites in Brazil and Chile dating up to 7,000 years ago. Using established protocols for aDNA extraction we obtained samples showing high degradation as usually happens with this kind of material. The reconstructive polymerization pretreatment was essential to overcome the DNA degradation and the serial dilutions helped with to prevent polymerase chain reaction (PCR) inhibitors. Moreover, the random amplified polymorphic DNA-PCR has been shown to be a reliable technique for further experiments to recover specific aDNA sequences.

【 授权许可】

CC BY   
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