Random amplified polymorphic DNA analysis of DNA extracted from Trichuris trichiura (Linnaeus, 1771) eggs and its prospective application to paleoparasitological studies

Memórias do Instituto Oswaldo Cruz

Elaine Machado Martinez¹  Jorge Antonio Santos Correia¹  Erika Verissimo Villela²  Antonio Nascimento Duarte¹  Luiz Fernando Ferreira¹  Alexandre Ribeiro Bello² 

[1] ,Fiocruz Escola Nacional de Saúde Pública Departamento de Endemias Samuel PessoaRio de Janeiro RJ ,Brasil

关键词: random amplified polymorphic DNA analysis;    Trichuris trichiura;    paleoparasitology;   

DOI  :  10.1590/S0074-02762003000900011

来源: SciELO

【 摘 要 】

Random amplified polymorphic DNA analysis was applied to DNAs extracted from Trichuris trichiura eggs recovered from human fecal samples. Four out of 6 primers tested displayed 18 distinct and well defined polymorphic patterns, ranging from 650 to 3200 base pairs. These results, upon retrieval and DNA sequencing of some of these bands from agarose gels, might help in establishing T. trichiura specific genetic markers, not available yet, and an important step to design primers to be used in molecular diagnosis approaches.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License