Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphism

Memórias do Instituto Oswaldo Cruz

Roberta L Caldeira²  Omar S Carvalho²  Cristiane Lfg Mendonça²  Carlos Graeff-teixeira¹  Márcia Cf Silva¹  Renata Ben¹  Rafael Maurer¹  Walter S Lima¹  Henrique L Lenzi¹ 

[1] ,Centro de Pesquisas René Rachou-Fiocruz Laboratório de Helmintoses Intestinais Belo Horizonte MG ,Brasil

关键词: Angiostrongylus;    polymerase chain reaction;    restriction fragment length polymorphism;    second internal transcribed spacer;    cytochrome oxidase I;   

DOI  :  10.1590/S0074-02762003000800011

来源: SciELO

【 摘 要 】

Angiostrongylus cantonensis, A. costaricensis, and A. vasorum are etiologic agents of human parasitic diseases. Their identification, at present, is only possible by examining the adult worm after a 40-day period following infection of vertebrate hosts with the third-stage larvae. In order to obtain a diagnostic tool to differentiate larvae and adult worm from the three referred species, polymerase chain reaction-restriction fragment length polymorphism was carried out. The rDNA second internal transcribed spacer (ITS2) and mtDNA cytochrome oxidase I regions were amplified, followed by digestion of fragments with the restriction enzymes RsaI, HapII, AluI, HaeIII, DdeI and ClaI. The enzymes RsaI and ClaI exhibited the most discriminating profiles for the differentiation of the regions COI of mtDNA and ITS2 of rDNA respectively. The methodology using such regions proved to be efficient for the specific differentiation of the three species of Angiostrongylus under study.

【 授权许可】

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