In vitro susceptibility characteristics of Cryptococcus neoformans varieties from AIDS patients in Goiânia, Brazil

Memórias do Instituto Oswaldo Cruz

Orionalda De Fl Fernandes¹  Xisto S Passos¹  Lúcia Kh Souza¹  André Tb Miranda¹  Carlos Henrique Pv Cerqueira¹  Maria Do Rosário R Silva¹ 

[1] ,Universidade Federal de Goiás Instituto de Patologia Tropical e Saúde Pública Goiânia GO ,Brazil

关键词: serotypes;    antifungal susceptibility;    Cryptococcus neoformans;    Goiânia;    Brazil;   

DOI  :  10.1590/S0074-02762003000600022

来源: SciELO

【 摘 要 】

Sixty clinical isolates of Cryptococcus neoformans from AIDS from Goiânia, state of Goiás, Brazil, were characterized according to varieties, serotypes and tested for antifungal susceptibility. To differentiate the two varieties was used L-canavanine-glycine-bromothymol blue medium and to separate the serotypes was used slide agglutination test with Crypto Check Iatron. The Minimal Inhibitory Concentration (MIC) of fluconazole, itraconazole, and amphotericin B were determined by the National Committee for Clinical Laboratory Standards macrodilution method. Our results identified 56 isolates as C. neoformans var. neoformans serotype A and 4 isolates as C. neoformans var. gattii serotype B. MIC values for C. neoformans var. gattii were higher than C. neoformans var. neoformans. We verified that none isolate was resistant to itraconazole and to amphotericin B, but one C. neoformans var. neoformans and three C. neoformans var. gattii isolates were resistant to fluconazole. The presence of C. neoformans var. gattii fluconazole resistant indicates the importance of determining not only the variety of C. neoformans infecting the patients but also measuring the MIC of the isolate in order to properly orient treatment.

【 授权许可】

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