Detection of hilA gene sequences in serovars of Salmonella enterica sufigbspecies enterica

Memórias do Instituto Oswaldo Cruz

Nora Cardona-castro²  Eliana Restrepo-pineda²  Margarita Correa-ochoa¹ 

[1] ,Instituto Colombiano de Medicina Tropical Laboratorio de Microbiología Sabaneta Antioquia ,Colombia

关键词: hilA gene sequences;    Salmonella serovars Typhi;    Enteritidis;    Choleraesuis;    Paratyphi A and B;    Pullorum;   

DOI  :  10.1590/S0074-02762002000800016

来源: SciELO

【 摘 要 】

hilA gene promoter, component of the Salmonella Pathogenicity Island 1, has been found in Salmonella serovar Typhimurium, being important for the regulation of type III secretion apparatus genes. We detected hilA gene sequences in Salmonella serovars Typhi, Enteritidis, Choleraesuis, Paratyphi A and B, and Pullorum, by polymerase chain reaction (PCR) and hybridization techniques. The primers to carry out PCR were designed according to hilA sequence. A low stringency hybridization with the probe pVV441 (hilA open-reading-frame plasmid) was carried out. To find hilA gene sequences in other Salmonella sp. suggest that these serovars could have similar sequences of this kind of virulence genes.

【 授权许可】

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