Derris (Lonchocarpus) urucu (Leguminosae) Extract Modifies the Peritrophic Matrix Structure of Aedes aegypti (Diptera:Culicidae)

Memórias do Instituto Oswaldo Cruz

Desiely Silva Gusmão²  Valéria Páscoa²  Leda Mathias¹  Ivo José Curcino Vieira¹  Raimundo Braz-filho¹  Francisco José Alves Lemos² 

[1] ,Universidade Estadual do Norte Fluminense Centro de Biociências e Biotecnologia Laboratório de Biotecnologia

关键词: peritrophic matrix;    larvicide;    Aedes aegypti;    Derris urucu;    plant extract;    insecticide;   

DOI  :  10.1590/S0074-02762002000300017

来源: SciELO

【 摘 要 】

Aqueous suspension of ethanol extracts of Derris (Lonchocarpus) urucu (Leguminosae), collected in the state of Amazonas, Brazil, were tested for larvicidal activity against the mosquito Aedes aegypti (Diptera:Culicidae). The aim of this study was to observe the alterations of peritrophic matrix in Ae. aegypti larvae treated with an aqueous suspension of D. urucu extract. Different concentrations of D. urucu root extract were tested against fourth instar larvae. One hundred percent mortality was observed at 150 µg/ml (LC50 17.6 µg/ml) 24 h following treatment. In response to D. urucu feeding, larvae excreted a large amount of amorphous feces, while control larvae did not produce feces during the assay period. Ultrastructural studies showed that larvae fed with 150 µg/ml of D. urucu extract for 4 h have an imperfect peritrophic matrix and extensive damage of the midgut epithelium. Data indicate a protective role for the peritrophic matrix. The structural modification of the peritrophic matrix is intrinsically associated with larval mortality.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License