Ultrastructural, Antigenic and Physicochemical Characterization of the Mojuí dos Campos (Bunyavirus) Isolated from Bat in the Brazilian Amazon Region

Memórias do Instituto Oswaldo Cruz

Ana Lm Wanzeller²  José Ap Diniz²  Maria Lc Gomes²  Ana Cr Cruz²  Manoel Cp Soares²  Wanderley De Souza¹  Amélia Pa Travassos Da Rosa¹  Pedro Fc Vasconcelos² 

[1] ,Instituto Evandro ChagasBelém PA ,Brasil

关键词: arbovirus;    Mojuí dos Campos virus;    characterization;    ultrastructure;    protein kinetics;    Amazon region;    Brazil;   

DOI  :  10.1590/S0074-02762002000300005

来源: SciELO

【 摘 要 】

The Mojuí dos Campos virus (MDCV) was isolated from the blood of an unidentified bat (Chiroptera) captured in Mojuí dos Campos, Santarém, State of Pará, Brazil, in 1975 and considerated to be antigenically different from other 102 arboviruses belonging to several antigenic groups isolated in the Amazon region or another region by complement fixation tests. The objective of this work was to develop a morphologic, an antigenic and physicochemical characterization of this virus. MDCV produces cytopathic effect in Vero cells, 24 h post-infection (p.i), and the degree of cellular destruction increases after a few hours. Negative staining electron microscopy of the supernatant of Vero cell cultures showed the presence of coated viral particles with a diameter of around 98 nm. Ultrathin sections of Vero cells, and brain and liver of newborn mice infected with MDCV showed an assembly of the viral particles into the Golgi vesicles. The synthesis kinetics of the proteins for MDCV were similar to that observed for other bunyaviruses, and viral proteins could be detected as early as 6 h p.i. Our results reinforce the original studies which had classified MDCV in the family Bunyaviridae, genus Bunyavirus as an ungrouped virus, and it may represent the prototype of a new serogroup.

【 授权许可】

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