Comparison between the Counter Immunoelectrophoresis Test and Mouse Neutralization Test for the Detection of Antibodies against Rabies Virus in Dog Sera

Memórias do Instituto Oswaldo Cruz

Luzia Helena Queiroz Da Silva¹  César Eduardo Bissoto¹  Cristiano De Carvalho¹  Tereza Cristina Cardoso¹  Devani M Pinheiro¹  Silvia Helena Venturoli Perri¹ 

[1],Universidade Estadual Paulista Curso de Medicina Veterinária Produção e Saúde AnimalAraçatuba SP ,Brasil

关键词: rabies;    rabies antibodies;    mouse neutralization test;    counterimmunoelectrophoresis test;   

DOI  :  10.1590/S0074-02762002000200020

来源: SciELO

【 摘 要 】

The detection of rabies antibodies is extremely valuable for epidemiological studies, determination of immune status in man, animals, and for the diagnosis of the disease. Several serological procedures have been described for this purpose. The present study reports a comparison between counterimmunoelectrophoresis test (CIET) and mouse neutralization test (MNT) in the detection of antibodies against rabies virus from 212 serum samples of vaccinated dogs. The agreement between both techniques was 79.7% and a significative association was demonstrated. The correlation coefficients between MNT and the CIET titers was determined considering 88 samples showing positive results in both techniques [CIET = 2 and MNT = 5 (0.13 IU/ml)] and resulted r² = 0.7926 (p < 0.001). The performance of CIET system was technically simple, cheap and rapid, and thereby it could be useful for serological monitoring of dog vaccination campaigns as well as for individual analysis.

【 授权许可】

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