A new continuous cell line from the mosquito Psorophora confinnis (Diptera: Culicidae) and its susceptibility to infections with some arboviruses

Memórias do Instituto Oswaldo Cruz

Felio J Bello¹  Jaime A Rodríguez¹  Jesús Escovar¹  Víctor A Olano²  Alberto Morales²  Martha González²  Gloria Rey² 

[1] ,Universidad De La Salle Departamento de Biología y Química Bogotá D.C. ,Colombia

关键词: Psorophora confinnis;    cell line;    chromosomes;    isozymes;    arbovirus;   

DOI  :  10.1590/S0074-02762001000600022

来源: SciELO

【 摘 要 】

A new cell line, PC-0199-BR, was established from embryonated eggs of the mosquito Psorophora confinnis. To date (September 2000) it has had 62 continuous passages. This is the first report of a cell line of mosquitoes belonging to the genus Psorophora. Cell growth initially was achieved in the MM/VP12 medium, supplemented with 20% fetal bovine serum; however, the subcultures were later adapted to Grace's medium with 10% fetal bovine serum. Cell morphology in the primary cultures was heterogeneous; but later in the established cell line, the predominant cell type was epithelioid. Cultured cells were predominantly diploid (2n=6); however, chromosome abnormalities were observed in a small proportion of the cells in later passages. C and G band patterns were also determined in the karyotype. The cell line isozyme profiles coincided with pupae and adult samples of the species taken from the same colony. A preliminary arbovirus susceptibility study for the cell line was undertaken. No evidence was observed of contamination of the cell line with bacteria, fungi or mycoplasma.

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