Identification of a differentially expressed mRNA in axenic Leishmania panamensis amastigotes

Memórias do Instituto Oswaldo Cruz

José Arturo Gutiérrez¹  Fabiola Puentes¹  Alberto Moreno¹  Manuel Elkin Patarroyo¹  Luis Angel Murillo¹ 

[1] ,Universidad Nacional de Colombia Hospital San Juan de Dios Instituto de InmunologiaSantafé de Bogotá,Colombia

关键词: differential display;    axenic amastigotes;    Leishmania panamensis;    mitogen activated protein kinase;   

DOI  :  10.1590/S0074-02762001000600017

来源: SciELO

【 摘 要 】

Differential display technique was applied in order to identify transcripts which are present in axenic amastigotes but not in promastigotes of the Leishmania panamensis parasites. One of them was cloned and the sequence reveals an open reading frame of 364 amino acids (aprox. 40 kDa). The deduced protein is homologous to the serine/threonine protein kinases and specially to the mitogen activates protein kinases from eukaryotic species. Southern blot analysis suggest that this transcript, named lpmkh, is present in the genome of the parasite as a single copy gene. These results could imply that lpmkh could be involved in the differentiation process or the preservation of amastigotes in axenic conditions.

【 授权许可】

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