A α-glycerophosphate dehydrogenase is present in Trypanosoma cruzi glycosomes

Memórias do Instituto Oswaldo Cruz

Jl Concepcion¹  H Acosta¹  W Quiñones¹  M Dubourdieu¹ 

[1] ,Universidad de Los Andes Facultad de Ciencias Centro de Ingenería GenéticaMérida,Venezuela

关键词: α -glycerophosphate dehydrogenase;    glycosome;    Trypanosoma cruzi;   

DOI  :  10.1590/S0074-02762001000500021

来源: SciELO

【 摘 要 】

α-glycerophosphate dehydrogenase (α-GPDH-EC.1.1.1.8) has been considered absent in Trypanosoma cruzi in contradiction with all other studied trypanosomatids. After observing that the sole malate dehydrogenase can not maintain the intraglycosomal redox balance, GPDH activity was looked for and found, although in very variable levels, in epimastigotes extracts. GPDH was shown to be exclusively located in the glycosome of T. cruzi by digitonin treatment and isopycnic centrifugation. Antibody against T. brucei GPDH showed that this enzyme seemed to be present in an essentially inactive form at the beginning of the epimastigotes growth. GPDH is apparently linked to a salicylhydroxmic-sensitive glycerophosphate reoxidizing system and plays an essential role in the glycosome redox balance.

【 授权许可】

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