C-banding and FISH in chromosomes of the blow flies Chrysomya megacephala and Chrysomya putoria (Diptera, Calliphoridae)

Memórias do Instituto Oswaldo Cruz

Patricia P Parise-maltempi²  Rita Mp Avancini¹ 

[1] ,Universidade Estadual de Campinas Instituto de Biologia Departamento de Biologia Celular

关键词: Chrysomya;    karyotype;    NOR;    heterochromatin;    blow flies;   

DOI  :  10.1590/S0074-02762001000300015

来源: SciELO

【 摘 要 】

The blow flies Chrysomya putoria and C. megacephala have 2n=12 chromosomes, five metacentric pairs of autosomes and an XX/XY sex chromosome pair. There are no substantial differences in the karyotype morphology of these two species, except for the X chromosome which is subtelocentric in C. megacephala and metacentric in C. putoria and is about 1.4 times longer in C. putoria. All autosomes were characterized by the presence of a C band in the pericentromeric region; C. putoria also has an interstitial band in pair III. The sex chromosomes of both species were heterochromatic, except for a small region at the end of the long arm of the X chromosome. Ribosomal genes were detected in meiotic chromosomes by FISH and in both species the NOR was located on the sex chromosomes. These results confirm that C. putoria was the species introduced into Brazil in 1970s, and not C. chloropyga as formerly described.

【 授权许可】

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