Mitochondrial DNA variation of Triatoma infestans populations and its implication on the specific status of T. melanosoma

Memórias do Instituto Oswaldo Cruz

Fernando A Monteiro²  Ruben Pérez¹  Francisco Panzera¹  Jean-pierre Dujardin¹  Cleber Galvão¹  Deyse Rocha¹  Francois Noireau¹  Chris Schofield¹  Charles B Beard² 

[1] ,Centers for Disease Control and Prevention Atlanta ,USA

关键词: Triatoma infestans;    Triatoma melanosoma;    mitochondrial DNA;    cytogenetics;    allozymes;    phylogeny;    molecular systematics;    Chagas disease vectors;   

DOI  :  10.1590/S0074-02761999000700037

来源: SciELO

【 摘 要 】

DNA sequence comparison of 412 base-pairs fragments of the mitochondrial cytochrome B gene was used to infer the genetic structure of nine geographical Triatoma infestans populations and their phylogenetic relationship with T. melanosoma and T. brasiliensis. T. infestans and T. melanosoma were compared by morphometry, allozyme and cytogenetic analyses, as well as subjected to reciprocal crosses, in order to clarify the taxonomic status of the latter. No differences were found to distinguish the two species and the crosses between them yielded progeny. T. infestans populations presented four haplotypes that could be separated in two clusters: one formed by the samples from Bolivia (Andes and Chaco) and the other formed by samples from Argentina and Brazil. Silvatic and domestic T. infestans populations from Bolivia (Andes) were genetically identical.

【 授权许可】

CC BY   
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