Properties of ß-lactamase from Neisseria gonorrhoeae

Memórias do Instituto Oswaldo Cruz

Marta C De Castillo¹  Fernando Sesma¹  Olga M De Nader¹  Aida P De Ruiz Holgado¹ 

[1] ,Universidad Nacional de Tucumán

关键词: Neisseria gonorrhoeae;    b-lactamase;    enzyme activity;   

DOI  :  10.1590/S0074-02761998000200020

来源: SciELO

【 摘 要 】

ß-lactamase activity was studied in Neisseria gonorrhoeae strains. Optimum temperature was found to be 37°C. The enzyme was inactivated at temperatures higher than 60°C, but remained active during storage at low temperatures (4°C, -30°C and -70°C) for two months. Enzyme activity was observed within a pH range of 5.8-8.0, while the optimum pH was 7.0-7.2. Addition of Ni2+, Fe2+, Fe3+, Mn2+ and p-chloromercurybenzoate to the reaction buffer exerted a negative effect upon the activity, whereas Hg2+ and ethylene diamine tetra-acetic acid produced complete inhibition. These results would indicate the presence of -SH groups at the catalytic site of the enzyme.

【 授权许可】

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