Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener

Memórias do Instituto Oswaldo Cruz

I Ferrari²  H Lorenzi²  Mr Santos¹  S Brandariz²  Jm Requena¹  A Schijman²  M Vázquez²  Jf Da Silveira¹  C Ben-dov²  C Medrano²  S Ghío²  P López Bergami²  I Cano¹  B Zingales¹  Tp Urmenyi¹  E Rondinelli¹  A González¹  A Cortes¹  Mc Lopez¹  Mc Thomas¹  C Alonso¹  Jl Ramírez¹  Ma Chiurrillo¹  R Rangel Aldao¹  A Brandão¹  W Degrave¹  V Perrot¹  M Saumier¹  A Billaut¹  D Cohen¹  D Le Paslier¹  Mj Levin² 

[1] ,Instituto de Investigaciones en Ingeniería Genética y Biología Molecular

关键词: Trypanosoma cruzi;    genome project;    physical map;    Yac library;    BAC library;    sequence tagged sites (STS);    expressed sequence tags (EST);   

DOI  :  10.1590/S0074-02761997000600021

来源: SciELO

【 摘 要 】

Strategies to construct the physical map of the Trypanosoma cruzi nuclear genome have to capitalize on three main advantages of the parasite genome, namely (a) its small size, (b) the fact that all chromosomes can be defined, and many of them can be isolated by pulse field gel electrophoresis, and (c) the fact that simple Southern blots of electrophoretic karyotypes can be used to map sequence tagged sites and expressed sequence tags to chromosomal bands. A major drawback to cope with is the complexity of T. cruzi genetics, that hinders the construction of a comprehensive genetic map. As a first step towards physical mapping, we report the construction and partial characterization of a T. cruzi CL-Brener genomic library in yeast artificial chromosomes (YACs) that consists of 2,770 individual YACs with a mean insert size of 365 kb encompassing around 10 genomic equivalents. Two libraries in bacterial artificial chromosomes (BACs) have been constructed, BACI and BACII. Both libraries represent about three genome equivalents. A third BAC library (BAC III) is being constructed. YACs and BACs are invaluable tools for physical mapping. More generally, they have to be considered as a common resource for research in Chagas disease

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