Conjugation by Mosquito Pathogenic Strains of Bacillus sphaericus

Memórias do Instituto Oswaldo Cruz

Margarita Correa¹  Allan A Yousten¹ 

关键词: Bacillus sphaericus;    mosquito pathogen;    bacterial conjugation;   

DOI  :  10.1590/S0074-02761997000300020

来源: SciELO

【 摘 要 】

A mosquito pathogenic strain of Bacillus sphaericus carried out the conjugal transfer of plasmid pAMß1 to other strains of its own and two other serotypes. However, it was unable to conjugate with mosquito pathogens from three other serotypes, with B. sphaericus of other DNA homology groups or with three other species of Bacillus. Conjugation frequency was highest with a strain having an altered surface layer (S layer). Conjugal transfer of pAMß1 was not detected in mosquito larval cadavers. B. sphaericus 2362 was unable to mobilize pUB110 for transfer to strains that had served as recipients of pAMß1. These observations suggest that it is unlikely that genetically engineered B. sphaericus carrying a recombinant plasmid could pass that plasmid to other bacteria

【 授权许可】

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