The haemoculture of Trypanosoma minasense chagas, 1908

Memórias do Instituto Oswaldo Cruz

Mariangela Ziccardi¹  Ricardo Lourenço-de-oliveira¹  Ricardo Nogueira² 

[1] ,Instituto Oswaldo Cruz Departamento de Protozoologia Laboratório de Imunidade Celular e HumoralRio de Janeiro RJ ,Brasil

关键词: Trypanosoma minasense;    neotropical primates;    trypanosomes;    marmosets;    trypanosomatidae flagellates;    culture;    Brazil;   

DOI  :  10.1590/S0074-02761996000400019

来源: SciELO

【 摘 要 】

Trypanosoma minasense was isolated for the first time in blood axenic culture from a naturally infected marmoset, Callithrix penicillata, from Brazil. The parasite grew profusely in an overlay of Roswell Park Memorial Institute medium plus 20% foetal bovine serum, on Novy, McNeal and Nicolle medium (NNN) , at 27°C, with a peak around 168 hr. The morphometry of cultural forms of T. minasense, estimates of cell population size and comparative growth in four different media overlays always with NNN, were studied. The infectivity of cultural forms to marmosets (C. penicillata and C. jacchus) and transformation of epimastigotes into metacyclic-like forms in axenic culture in the presence of chitin derivates (chitosan) were evaluated.

【 授权许可】

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