Antibody isotypes, including IgG subclasses, in Ecuadorian patients with pulmonary Paragonimiasis

Memórias do Instituto Oswaldo Cruz

Angel Guevara E.²  Juan Carlos Vieira G.¹  Edmundo Araujo N.¹  Manuel Calvopiña H.¹  Ronald H. Guderian¹  Yves Carlier¹ 

[1] ,Universite Libre de Bruxelles Laboratoire de Parasilogie Bruxelles,Belgium

关键词: cuador;    pulmonary paragonimiasis;    IgM;    IgG;    IgG subclasses;   

DOI  :  10.1590/S0074-02761995000400012

来源: SciELO

【 摘 要 】

An ELISA test was developed to detect Paragonimus-specific antibodies, including IgG subclasses, using P. mexicanus crude water-soluble antigens. The test was standardized to detect antibodies in sera of Ecuadorian patients with pulmonary paragonimiasis and negative controls from the endemic area. The detected mean levels of IgG (0.753, SEM: 0.074) and IgM (0.303, SEM: 0.033) were significantly elevated (P<0.05). Within the IgG subclasses, IgG4 showed the highest detected mean level (0.365, SEM: 0.116) and the other three subclasses showed considerably lower mean levels (IgG1, 0.186 SEM: 0.06; IgG2, 0.046 SEM: 0.01; IgG3, 0.123 SEM: 0.047). The number of P. mexicanus eggs found in sputum of infected individuals showed a positive correlation with the level of antibodies detected for IgM, IgG and its subclasses (P<0.001). The relevance of these findings in Ecuadorian patients suffering from pulmonary paragonimiasis is discussed.

【 授权许可】

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