DNA polymorphism of schistosomes and their snail hosts

Memórias do Instituto Oswaldo Cruz

Andrew J. G. Simpson²  Emmanuel Dias Neto²  Teofania H. D. A. Vidigal²  Heloísa B. Pena¹  Omar S. Carvalho²  Sergio D. J. Pena¹ 

[1] ,FIOCRUZ Centro de Pesquisas René Rachou Belo Horizonte,Brasil

关键词: schistosomes;    Biomphalaria glabrata;    DNA;    polymorphisms;    PCR;   

DOI  :  10.1590/S0074-02761995000200014

来源: SciELO

【 摘 要 】

Analysis of the genomes of schistosomes and one of their intermediate hosts, Biomphalaria glabrata, using Random Amplified Polymorphic DNA (RAPD) demonstrated that intraspecific genetic polymorphism in the parasite is limited but in the snail is highly pronounced. This suggests an important role for the snail in the determination of the epidemiology of the disease. In addition to their intraspecific stability, schistosome derived RAPDs exhibit a high level of interspecific polymorphism and are thus ideal for the construction of phylogenetic trees. For the detection of intraspecific polymorphisms extensive variation in the mitochondrial DNA is being exploited for the development of a PCR based test for Schistosoma mansoni. Gene level polymorphisms are being analyzed by Low Stringency Single Specific Primer PCR.

【 授权许可】

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