Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review

Memórias do Instituto Oswaldo Cruz

Win Degrave²  Octavio Fernandes¹  David Campbell¹  Marcelo Bozza¹  Ulisses Lopes¹ 

[1] ,Instituto Oswaldo Cruz Departamento de Bioquímica e Biologia Molecular Rio de Janeiro,Brasil

关键词: Leishmania;    polymerase chain reaction;    hybridization;    molecular probes;    mini-exon gene;    minicircle;    kDNA;   

DOI  :  10.1590/S0074-02761994000300032

来源: SciELO

【 摘 要 】

The use of molecular tools to detect and type Leishmania species in humans, reservoirs or sandflies has been pursued using different approaches. The polymerase chain reaction provided sensitivity to case this task, since the use of hybridization procedures alone employing specifics probes is hampered due to the low detection limit. In this report, we describe the different molecular targets used in our laboratory, aiming at the detection and specific typing of these protozoa. Different kits based on hybridization assays and PCR amplification using kinetoplast and nuclear targets are described and the results obtained from their use are reported.

【 授权许可】

CC BY   
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