Use of an indirect haemagglutination test, for the detection of Clostridium perfringens type A enterotoxin

Memórias do Instituto Oswaldo Cruz

Marlene B. Serafim¹  Isildinha A. G. Colli¹  Lucila Costallat Ricci¹  Antonio F. Pestana De Castro¹ 

[1] ,Universidade Estadual de Campinas Instituto de Biologia Departamento de MicrobiologiaCampinas,Brasil

关键词: Clostridum perfrigens;    enterotoxin;    indirect;    haemagglutination;   

DOI  :  10.1590/S0074-02761991000100005

来源: SciELO

【 摘 要 】

An indirect haemagglutination (IH) test is described for the detection of Clostridium perfringenstype A enterotoxin, produced by strains isolated from human cases of food poisoning and from contaminated food. Though no strict relationship could be observed between titers in the IH test and the time it took mice to die from the intravenous inoculation of mice (IIM), results of the supernatants examined by both methods demonstrated that the IH test was more sensitive than the ILM one. No unspecific reaction was obtanined int he IH wirh a negative control and the inhibitions of the IH and IIM tests by specific antiserum against C. perfringens enterotoxin showed that the IH test is very spcific. The IH assay is recommended for its sensitivity and easy performance by less-equipped laboratories, by these and other data.

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