Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies

Memórias do Instituto Oswaldo Cruz

Luiz Tadeu Moraes Figueiredo²  Rita Maria Ribeiro Nogueira¹  Silvia Maria Baêta Cavalcanti¹  Hermann Schatzmayr¹  Amélia Travassos Da Rosa¹ 

[1] ,USP Faculdade de Medicina de Ribeirão Preto Departamento de Clínica MédicaRibeirão Preto,Brasil

关键词: Mayaro;    alphavirus;    arbovirus;    enzyme immunoassay;    Mayaro;    alphavirus;    arbovirus;    teste imuno-enzimático;   

DOI  :  10.1590/S0074-02761989000300003

来源: SciELO

【 摘 要 】

This paper presents the evaluation of an enzyme immunoassay in which Mayaro virus-infected cultured cells ara used as antigen (EIA-ICC) and an IgM antibody capture ELISA (MAC-ELISA) for Mayaro serologic diagnosis using 114 human sera obtained during a Mayaro outbreak occurred in Bolivia, in 1987. Results were compared with those obtained by haemagglutination-inhibition test (HAI). MAC-ELISA was the most sensitive technique for anti-Mayaro IgM detection. MAC-ELISA was twice sensitive as IgM EIA-ICC. The data shows that MAC-ELISA is a practical and valid technique for diagnosis of recent mayaro infection. IgG-ICC showed hight sensitivity and high specificity compared to HAI. The combination of anti-Mayaro IgG and IgM EIA-ICC results presented the highest sensitivity of the study. Anti-Mayaro IgG and IgM simultaneous detection by ELISA-ICC can be used for recent infection diagnosis (in spite of a less sensitive IgM detection than by MAC-ELISA), for surveillance and sero-epidemiologic studies, and for studies of IgG and IgM responses to Mayaro infection.

【 授权许可】

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