Memórias do Instituto Oswaldo Cruz | |
Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies | |
Luiz Tadeu Moraes Figueiredo2  Rita Maria Ribeiro Nogueira1  Silvia Maria Baêta Cavalcanti1  Hermann Schatzmayr1  Amélia Travassos Da Rosa1  | |
[1] ,USP Faculdade de Medicina de Ribeirão Preto Departamento de Clínica MédicaRibeirão Preto,Brasil | |
关键词: Mayaro; alphavirus; arbovirus; enzyme immunoassay; Mayaro; alphavirus; arbovirus; teste imuno-enzimático; | |
DOI : 10.1590/S0074-02761989000300003 | |
来源: SciELO | |
【 摘 要 】
This paper presents the evaluation of an enzyme immunoassay in which Mayaro virus-infected cultured cells ara used as antigen (EIA-ICC) and an IgM antibody capture ELISA (MAC-ELISA) for Mayaro serologic diagnosis using 114 human sera obtained during a Mayaro outbreak occurred in Bolivia, in 1987. Results were compared with those obtained by haemagglutination-inhibition test (HAI). MAC-ELISA was the most sensitive technique for anti-Mayaro IgM detection. MAC-ELISA was twice sensitive as IgM EIA-ICC. The data shows that MAC-ELISA is a practical and valid technique for diagnosis of recent mayaro infection. IgG-ICC showed hight sensitivity and high specificity compared to HAI. The combination of anti-Mayaro IgG and IgM EIA-ICC results presented the highest sensitivity of the study. Anti-Mayaro IgG and IgM simultaneous detection by ELISA-ICC can be used for recent infection diagnosis (in spite of a less sensitive IgM detection than by MAC-ELISA), for surveillance and sero-epidemiologic studies, and for studies of IgG and IgM responses to Mayaro infection.
【 授权许可】
CC BY
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202103040043828ZK.pdf | 267KB | download |