Evaluation of antibody responses in american visceral leishmaniasis by ELISA and immunoblot

Memórias do Instituto Oswaldo Cruz

Thomas G. Evans²  Edward C. Krug²  Mary E. Wilson¹  Antonio Wilson Vasconcelos¹  Joaquim Eduardo De Alencar¹  Richard D. Pearson² 

[1] ,University of Virginia School of Medicine Departments of Medicine and Pathology. Divisions of Geographic Medicine and Infectious DiseasesCharlottesville,USA

关键词: Visceral leishmaniasis;    Leishmania donovani chagasi;    ELISA;    immunoblot;    leishmaniose visceral;    Leishmania donovani chagasi;    ELISA;    "Immunoblot";   

DOI  :  10.1590/S0074-02761989000200003

来源: SciELO

【 摘 要 】

American visceral leishmaniasis (AVL) is an important disease among children of northeast Brazil. In order to characterize antibody responses during AVL, sera of hospitalized patients were analyzed by ELISA and Western blot using a Leishmania chagasi antigen preparation. The ELISA was positive (asorbance [greater than or equals to] 0.196) at a serum dilution of 1:1024 in all patients at presentation, and fell to ward control levels over the following year. Only one of 72 control subjects tested positive, and that donor had a sibling with AVL. Immunoblots of the patients' sera recognized multiple bands, the most frequent of which were at approximately 116 kDa, 70 kDa, and 26 kDa. Less frenquently observed were bands at approximately 93 kDa, 74 kDa, 62 kDa, 46 kDa and 32 kDa. The ELISA responses and patterns of banding were distinctive for AVL, and could be used to differentiate patients with AVL from those with Chagas' disease of cutaneous leishmaniasis. Sera from six AVL patients followed for up to six weeks after treatment identified no new bands. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of surface iodinated parasite proteins showed one major band and four minor bands, whereas SDS-PAGE of biotinylated prarasite proteins revealed a banding pattern similar to those of patient sera. AVL appears to produce characteristic immunoblot patterns which can be used along with a sensitive screening ELISA to diagnose AVL.

【 授权许可】

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