Biological aspects of the DM28C clone of Trypanosoma cruzi after metacylogenesis in chemically defined media

Memórias do Instituto Oswaldo Cruz

Victor T. Contreras²  Tania C. De Araújo-jorge¹  Myrna C. Bonaldo²  Neide Thomaz¹  Helena S. Barbosa¹  Maria De Nazareth S. L. De Meirelles¹  Samuel Goldenberg² 

[1] ,Instituto Oswaldo Cruz Departamento de Bioquímica e Biologia Molecular Rio de Janeiro,Brasil

关键词: Trypanosoma cruzi;    metacyclogenesis;    Chaga's disease;    muscle cells;    Trypanosoma cruzi;    metaciclogênese;    doença de Chagas;    células de músculo;   

DOI  :  10.1590/S0074-02761988000100016

来源: SciELO

【 摘 要 】

The biological characterization of the Trypanosoma cruzi clone Dm 28c in terms of its growth in LIT medium, cell-cycle, infectivity to mice and interaction with professional and non-professional phagocytic cells shows that it behaves as a bona fide T. cruzi representant. The biological properties of this myotropic clone do not change according to the origin of thetrypomastigote forms (i. e., from triatomines, infected mice, cell-culture or from the chemically defined TAUP and TAU3AAG media). In addition Dm 28c metacyclic trypomastigotes from TAU3AAG medium display a high infectivity level to fibroblasts and muscle cells. Experiments on binding of cationized ferritin to trypomastigotes surface show the existence of cap-like structures of ferritin in regions near the kinetoplast. However the nature and role of these anionic sites remain to be determined. The results indicate that metacyclic trypomastigotes from Dm 28c clone obtained under chemically defined conditions reproduce the biological behaviour of T. cruzi, rendering this system very suitable for the study of cell-parasite interactions and for the isolation of trypanosome relevant macromolecules.

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