Leishmania mexicana amazonensis: heterogeneity in 5-nucleotidase and peroxidase activities of mononuclear phagocytes during in vivo and in vitro infection

Memórias do Instituto Oswaldo Cruz

Suzana Côrte-real²  Gabriel Grimaldi Junior¹  Maria De Nazareth Leal De Meirelles² 

[1] ,Instituto Oswaldo Cruz Departamento de Ultraestrutura e Biologia Celular Rio de Janeiro,Brasil

关键词: Leishmania mexicana amazonensis (L. m. amazonensis);    immature mononuclear phagocyte;    mature macrophages;    periotoneal macrophages;    cutaneous lesions;    5'Nucleotidase (E. C. 3. 1. 3. 5);    Peroxidase (E. C. 1. 11. 1. 7);    Leishmania mexicana amazonensis (L. m. amazonensis);    fagócito mononuclear imaturo;    macrófago maturo;    macrófago peritoneal;    lesões cutâneas;    5'Nucleotidase (E. C. 3. 1. 3. 5);    Peroxidase (E. C. 1. 11. 1. 7);   

DOI  :  10.1590/S0074-02761988000100015

来源: SciELO

【 摘 要 】

The degree of maturation of cells of the Mononuclear Phagocyte System (MPS), during in vivo and in vitro infection by Leishmania mexicana amazonenesis, was evaluated in this study. The macrophages' differentiation was assayed by cytochemical characterization at the ultrastrctural level, using two well-established markers: 5'-nucleotidase enzyme activity, for revealing the mature cells, and the peroxidase activity present in the cell granules to demonstrate immature mononuclear phagocytes. only a few mcrophages, demonstrating 5'-nucleotidase positive reaction in both the plasma membrane and within their cytoplasmic vesicles, were found scattered in the chronic inflammation at the L. m. amazonensis lesions in albino mice. However, by the peroxidase activity analysis, we were also able to demonstrate the presence of immature MPS cells, which predominate, together with parasitized vacuolated macrophages, in chronic lesions induced in this systemby L. m. amazonensis. The implications of these results on the pathogenesis of murine cutaneous leishmaniasis are discussed.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License