The performance of an in-house nested-PCR technique for pleural tuberculosis diagnoses

Revista da Sociedade Brasileira de Medicina Tropical

Lilian Maria Lapa Montenegro¹  Bruno Cesar Da Silva¹  Juliana Figueiredo Da Costa Lima¹  Heidi Lacerda Alves Da Cruz¹  Rosana De Albuquerque Montenegro¹  Fernando Luiz Cavalcanti Lundgren¹  Alfredo Pereira Leite De Albuquerque Filho¹  Haiana Charifker Schindler¹ 

关键词: Pleural tuberculosis;    Extrapulmonary tuberculosis;    Nested PCR diagnosis;    Mycobacterium tuberculosis;   

DOI  :  10.1590/0037-8682-0127-2013

来源: SciELO

【 摘 要 】

IntroductionThis study evaluated the performance of an in-house nested-PCR system for the detection of the Mycobacterium tuberculosis complex in pleural fluid, blood and urine samples from pleural effusion tuberculosis patients by health services physicians in Pernambuco, Brazil.Methods A prospective double-blind study with 37 hospitalized patients of both sexes, aged over 15, was used to investigate the diagnosis of pleural effusion. The criteria used to define the cases included the demonstration of bacillus in biological samples by smear or culture or by a granulomatous finding in the histopathological examination, associated with an evident response to specific treatments to each clinical situation. Pleural fluid, blood and urine samples were collected and subjected to routine tests and the nested PCR technique to assess for M. tuberculosis amplification.Results In total, 37 pleural effusion patients took part in the study, of whom 19 (51.3%) had tubercular etiologies and 18 (48.7%) had etiologies from other causes. When the pleural fluid, blood and/or urine sample in-house nested-PCR sensitivities were evaluated simultaneously, the results were positive regardless of the biological specimen (the sensitivity was 84.2%); however, when the blood and/or urine samples were analyzed together, the sensitivity was 72.2%. When the pleural fluid samples were evaluated alone, the sensitivity was only 33.3%.ConclusionsThe performance of the diagnostic pleural tuberculosis nested-PCR was directly related to the diversity of the samples collected from the same patient. Additionally, this study may identify a need to prioritize non-invasive blood and urine collection for this diagnosis.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License