期刊论文详细信息
Revista da Sociedade Brasileira de Medicina Tropical
Different culture media containing methyldopa for melanin production by Cryptococcus species
Ralciane De Paula Menezes2  Mário Paulo Amante Penatti1  Reginaldo Dos Santos Pedroso1 
[1] ,Universidade Federal de Uberlândia Instituto de Ciências Biomédicas Uberlândia,MG
关键词: Cryptococcus sp;    Melanin;    Culture media;    Methyldopa;    Cryptococcus sp;    Melanina;    Meios de cultura;    Metildopa;   
DOI  :  10.1590/S0037-86822011000500012
来源: SciELO
PDF
【 摘 要 】

INTRODUCTION: Melanin production by species of Cryptococcus is widely used to characterize C. neoformans complex in mycology laboratories. This study aims to test the efficacy of methyldopa from pharmaceutical tablet as a substrate for melanin production, to compare the production of melanin using different agar base added with methyldopa, and to compare the melanin produced in those media with that produced in Niger seed agar and sunflower seed agar by C. neoformans, C. laurentii, and C. albidus. Two isolates of each species, C. neoformans, C. laurentii, and C. albidus, and one of Candida albicans were used to experimentally detect conditions for melanin production. METHODS: The following media were tested: Mueller-Hinton agar (MHA), brain and heart infusion agar (BHIA), blood agar base (BAB), and minimal medium agar (MMA), all added with methyldopa, and the media Niger seed agar (NSA) and sunflower seed agar (SSA). RESULTS: All isolates grew in most of the culture media after 24h. Strains planted on media BAB and BHIA showed growth only after 48h. All isolates produced melanin in MMA, MHA, SSA, and NSA media. CONCLUSIONS: Methyldopa in the form pharmaceutical tablet can be used as a substrate for melanin production by Cryptococcus species; minimal medium plus methyldopa was more efficient than the BAB, MHA, and BHIA in the melanin production; and NSA and SSA, followed by MMA added with methyldopa, were more efficient than other media studied for melanin production by all strains studied.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License

【 预 览 】
附件列表
Files Size Format View
RO202103040037452ZK.pdf 1143KB PDF download
  文献评价指标  
  下载次数:10次 浏览次数:11次