Ultrastructural study on the morphological changes to male worms of Schistosoma mansoni after in vitro exposure to allicin

Revista da Sociedade Brasileira de Medicina Tropical

Caliandra Maria Bezerra Luna Lima²  Francisca Inês De Sousa Freitas¹  Liana Clébia Soares Lima De Morais²  Marília Gabriela Dos Santos Cavalcanti²  Lânia Ferreira Da Silva¹  Rafael José Ribeiro Padilha¹  Constância Gayoso Simões Barbosa¹  Fábio André Brayner Dos Santos¹  Luiz Carlos Alves¹  Margareth De Fátima Formiga Melo Diniz¹ 

[1] ,Universidade Federal da Paraíba Departamento de Fisiologia e Patologia João Pessoa PB

关键词: Garlic;    Schistosoma mansoni;    Scanning electron microscopy;    Alho;    Schistosoma mansoni;    Microscopia eletrônica de varredura;   

DOI  :  10.1590/S0037-86822011005000023

来源: SciELO

【 摘 要 】

INTRODUCTION: Garlic has a wide range of actions, including antibacterial, antiviral, antifungal, antiprotozoal and anthelmintic actions. This antiparasitic activity has been attributed to allicin, which is the main constituent of garlic. The present study aimed to investigate the in vitro activity of allicin on the tegument of adult Schistosoma mansoni worms using scanning electron microscopy. METHODS: Swiss Webster mice were infected with S. mansoni cercariae (100 per mouse) and sacrificed 50 days later to acquire the adult worms. These worms were collected by perfusion and placed in RPMI medium 1,640 at 37°C before transferring to RPMI media containing 0 (control), 5, 10, 15 and 20mg/mL of allicin, where they were incubated for 2h. The worms were fixed in 2.5% glutaraldehyde solution, washed twice, post-fixed in osmium tetroxide, washed twice and then dehydrated with ascending grades of ethanol. The samples were air-dried, mounted on stubs, gold coated in an ion sputtering unit and viewed using a scanning electron microscope. RESULTS: A concentration of 5mg/mL caused wrinkling in the tegument; a concentration of 10mg/mL resulted in changes to tubercles and loss or modification of spines. With 15 and 20mg/mL increasing damage to the tegument could be seen, such as vesicle formation and the presence of ulcers. CONCLUSIONS: These findings demonstrate the effect of allicin on adult S. mansoni worms and indicate that most of the changes occur at concentrations greater than that normally indicated for treatment.

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