Hydrophobic fraction of Taenia saginata metacestodes, rather than hydrophilic fraction, contains immunodominant markers for diagnosing human neurocysticercosis

Revista da Sociedade Brasileira de Medicina Tropical

Flávia De Assunção Gonçalves²  Gleyce Alves Machado²  Heliana Batista Oliveira²  Maria Teresa Nunes Pacheco Rezende¹  José Roberto Mineo²  Julia Maria Costa-cruz² 

[1] ,Federal University of Uberlândia Institute of Biomedical Sciences Department of Immunology, Microbiology and ParasitologyUberlândia MG

关键词: Neurocysticercosis;    Taenia solium;    Taenia saginata;    Heterologous antigen;    Detergent extraction;    Immunodiagnosis;    Neurocisticercose;    Taenia solium;    Taenia saginata;    Antígeno heterólogo;    Extração detergente;    Imunodiagnóstico;   

DOI  :  10.1590/S0037-86822010000300008

来源: SciELO

【 摘 要 】

INTRODUCTION: Considering that alternative antigens for diagnosing neurocysticercosis continue to be a challenge because of the increasing difficulty in obtaining parasites from naturally infected pigs for preparation of Taenia solium homologous antigen, the aim of the present study was to evaluate the detergent (D) and aqueous (A) fractions from saline extract of Taenia saginata metacestodes for diagnosing neurocysticercosis. METHODS: Taenia saginata was obtained from naturally infected bovines in the Triângulo Mineiro region, State of Minas Gerais, Brazil. The carcasses came from cold storage units and had been slaughtered in accordance with the inspection technique recommended by the Federal Inspection Service. The D and A fractions were obtained by using Triton X-114 (TX-114). Serum samples were obtained from 40 patients with a diagnosis of neurocysticercosis, 45 with other parasitic diseases and 30 from apparently normal individuals. IgG antibody levels were evaluated using the ELISA and immunoblotting assays. RESULTS: The ELISA sensitivity and specificity were 95% and 73.3%, when using saline extract; 95% and 82.6% for the D fraction; and 65% and 61.3% for the A fraction, respectively. The immunoblotting assay confirmed the ELISA results, such that the D fraction was more efficient than the other extracts, and the 70-68kDa component was immunodominant among neurocysticercosis patients. CONCLUSIONS: These results demonstrated that the D fraction from Taenia saginata metacestodes obtained using TX-114 can be used as a heterologous antigenic fraction in the immunoblotting assay for serologically diagnosing human neurocysticercosis, given its ability to select immunodominant antigens.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License