Phenetic studies on randomly amplified polymorphic DNA-polymerase chain reaction-variability of four geographical populations of Lutzomyia whitmani (Diptera: Psychodidae) in Brazil

Revista da Sociedade Brasileira de Medicina Tropical

Carina Margonari De Souza²  Consuelo Latorre Fortes-dias¹  Pedro Marcos Linardi¹  Edelberto Santos Dias² 

[1] ,Fundação Oswaldo Cruz Centro de Pesquisas René Rachou Laboratório de LeishmaniosesBelo Horizonte MG

关键词: Lutzomyia whitmani;    Sand fly;    Genetic variability;    RAPD-PCR;    Lutzomyia whitmani;    Flebotomíneo;    Variabilidade genética;    Reação em cadeia da polimerase;   

DOI  :  10.1590/S0037-86822004000200007

来源: SciELO

【 摘 要 】

Previous evaluation of the genetic variability of four biogeographical populations of Lutzomyia whitmani from known foci of cutaneous leishmaniasis in Brazil demonstrated two main spatial clusters: Corte de Pedra-BA, Ilhéus-BA and Serra de Baturité-CE in the first cluster, and Martinho Campos-MG in the second. Further analysis showed a high degree of homogeneity in Corte de Pedra population but not in the others, which presented a significant percentage of specimens displaced from their phenon of origin (discrepant individuals). In the present work we analyzed the frequencies of association coefficients in the matrixes of similarity per population of Lutzomyia whitmani from both sexes and the general phenograms obtained, in a more detailed study of those discrepant specimens. Populational stability was observed for Corte de Pedra population, whereas the three remaining populations showed varying degrees of heterogeneity and different displacements according to sex. Our results strongly suggested the existence of a genetic flow between the lineages North-South/North-East and Ilhéus/Serra do Baturité of Lutzomyia whitmani.

【 授权许可】

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