Pathogenicity of different rabies virus isolates and protection test in vaccinated mice

Revista do Instituto de Medicina Tropical de São Paulo

Elenice M.s. Cunha²  Alessandra F.c. Nassar²  Maria Do Carmo C.s.h. Lara²  Eliana C.m. Villalobos²  Go Sato¹  Yuki Kobayashi¹  Youko Shoji¹  Takuya Itou¹  Takeo Sakai¹  Fumio H. Ito¹ 

[1] ,Instituto Biológico de São Paulo Laboratório de Raiva e Encefalites Virais São Paulo SP ,Brasil

关键词: Rabies virus;    Pathogenicity;    Vaccine;   

DOI  :  10.1590/S0036-46652010000500002

来源: SciELO

【 摘 要 】

This study was aimed to evaluate and compare the pathogenicity of rabies virus isolated from bats and dogs, and to verify the efficacy of a commercial rabies vaccine against these isolates. For evaluation of pathogenicity, mice were inoculated by the intramuscular route (IM) with 500MICLD50/0.03mL of the viruses. The cross-protection test was performed by vaccinating groups of mice by the subcutaneous route and challenged through the intracerebral (IC) route. Isolates were fully pathogenic when inoculated by the IC route. When inoculated intramuscularly, the pathogenicity observed showed different death rates: 60.0% for the Desmodus rotundus isolate; 50.0% for dog and Nyctinomops laticaudatus isolates; 40.0% for Artibeus lituratus isolate; 9.5% Molossus molossus isolate; and 5.2% for the Eptesicus furinalis isolate. Mice receiving two doses of the vaccine and challenged by the IC route with the isolates were fully protected. Mice receiving only one dose of vaccine were partially protected against the dog isolate. The isolates from bats were pathogenic by the IC route in mice. However, when inoculated through the intramuscular route, the same isolates were found with different degrees of pathogenicity. The results of this work suggest that a commercial vaccine protects mice from infection with bat rabies virus isolates, in addition to a canine rabies virus isolate.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License