Evaluation of the human immunodeficiency virus type 1 and 2 antibodies detection in dried whole blood spots (dbs) samples

Revista do Instituto de Medicina Tropical de São Paulo

Andréa Cauduro De Castro²  Luiz Gustavo Dos Anjos Borges¹  Ricardo Da Silva De Souza¹  Melina Grudzinski¹  Pedro Alves D'azevedo¹ 

[1] ,Fundação Faculdade Federal de Ciências Médicas de Porto Alegre Programa de Pós-graduação em PatologiaRS ,Brasil

关键词: Filter paper;    HIV diagnosis;    DBS stability;   

DOI  :  10.1590/S0036-46652008000300004

来源: SciELO

【 摘 要 】

Human Immunodeficiency Vírus Type 1 and 2 antibodies detection was performed in 457 dried whole blood spots samples (S&S 903). Q-Preven HIV 1+2 was the screening test used. The results were compared with the gold standard serum tests by ELISA (Cobas Core e Axsym HIV1/2 gO) and imunofluorescence was the definitive confirmatory test. The samples were obtained from the Hospital Nossa Senhora da Conceição in Porto Alegre, RS - Brazil, through whole blood transfer to filter paper card and sent to Caxias do Sul, RS - Brazil where the tests were performed. The dried whole blood spot stability was evaluated with two different panels. The first one was composed of five negative and five positive samples stored at room temperature, 4 ºC, -20 ºC and -70 ºC, while the second was composed of two negative and three positive samples stored at 37 ºC (humidity <50%). Each sample was screened every week for six weeks. These measurement results didn't show variation during the study period. The detected sensibility was 100%, specificity was 99.6%, the positive predictive value was 99.5% and negative predictive values were 100%. The results demonstrated high performance characteristics, opening a new perspective of dried whole blood spot utilization in HIV screening diagnosis.

【 授权许可】

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