Diagnosis of abdominal angiostrongyliasis by PCR from sera of patients

Revista do Instituto de Medicina Tropical de São Paulo

Ana Cristina Arámburu Da Silva²  Carlos Graeff-teixeira²  Arnaldo Zaha¹ 

[1] ,PUCRS Faculdade de Biociências

关键词: Angiostrongylus costaricensis;    Eosinophilic gastroenteritis;    PCR;   

DOI  :  10.1590/S0036-46652003000500011

来源: SciELO

【 摘 要 】

Abdominal angiostrongyliasis is a zoonotic infection caused by an intra-vascular nematode parasitic of wild rodents, Angiostrongylus costaricensis. No parasitological diagnosis is currently available and immunodiagnosis presents several drawbacks. Primers constructed based on a congeneric species, A. cantonensis, were able to amplify a 232 bp fragment from serum samples of 3 patients with histopathological diagnosis. Extraction was better performed with DNAzol and the specificity of the primers was confirmed by Southern blot. This disease has been diagnosed with frequency in south of Brazil, thus, this method appears like the important and unpublished alternative to improve diagnostic of disease.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License