Evaluation of Etest and macrodilution broth method for antifungal susceptibility testing of Candida sp strains isolated from oral cavities of AIDS patients

Revista do Instituto de Medicina Tropical de São Paulo

Maria Do Rosário R. Silva²  Márcio R. Costa²  André T.b. Miranda²  Orionalda De F.l. Fernandes²  Carolina R. Costa²  Claudete R. De Paula¹ 

[1] ,Universidade Federal de Goiás Instituto de Patologia Tropical e Saúde Pública Goiânia GO ,Brasil

关键词: Etest;    Broth macrodilution;    Antifungal susceptibility tests;   

DOI  :  10.1590/S0036-46652002000300002

来源: SciELO

【 摘 要 】

A comparison of the Etest and the reference broth macrodilution susceptibility test for fluconazole, ketoconazole, itraconazole and amphotericin B was performed with 59 of Candida species isolated from the oral cavities of AIDS patients. The Etest method was performed according to the manufacturer's instructions, and the reference method was performed according to National Committee for Clinical Laboratory Standards document M27-A guidelines. Our data showed that there was a good correlation between the MICs obtained by the Etest and broth dilution methods. When only the MIC results at ± 2 dilutions for both methods were considered, the agreement rates were 90.4% for itraconazole, ketoconazole and amphotericin B and 84.6% for fluconazole of the C. albicans tested. In contrast, to the reference method, the Etest method classified as susceptible three fluconazole-resistant isolates and one itraconazole-resistant isolate, representing four very major errors. These results indicate that Etest could be considered useful for antifungal sensitivity evaluation of yeasts in clinical laboratories.

【 授权许可】

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