Dot-ELISA for the detection of anti-Cysticercus cellulosae antibodies in cerebrospinal fluid using a new solid phase (resin-treated polyester fabric) and Cysticercus longicollis antigens

Revista do Instituto de Medicina Tropical de São Paulo

Adelaide José Vaz²  Paulo Mutuko Nakamura¹  Mario E. Camargo¹  Eide Dias Camargo¹  A. Walter Ferreira¹ 

[1] ,Universidade de São Paulo Faculdade de Ciências-Farmacêuticas São Paulo,Brasil

关键词: Neurocysticercosis;    Dot-ELISA;    Cerebrospinal fluid;    Cysticercus cellulosae;    Cysticercus longicollis;    Resin-treated polyester fabric;   

DOI  :  10.1590/S0036-46651996000600001

来源: SciELO

【 摘 要 】

A dot-ELISA was developed for the detection of antibodies in CSF in the immunologic diagnosis of human neurocysticercosis, using antigen extracts of the membrane and scolex of Cysticercus cellulosae (M+S-Cc) and, alternately, membrane (M) and vesicular fluid (VF) of Cysticercus longicollis (Cl) covalently bound to a new solid phase consisting of polyester fabric treated with N-methylol-acrylamide resin (dot-RT). The test was performed at room temperature, with reduced incubation times and with no need for special care in the manipulation of the support. The sensitivity rates obtained were 95.1% for antigen Cc and 97.6% for antigen Cl. Specificity was 90.6% when Cc was used, and 96.9% and 100% when M-Cl and VF-Cl were used, respectively. No significant differences in titer were observed between tests carried out with homologous and heterologous antigens. The low cost and easy execution of the dot-RT test using antigen extracts of Cysticercus longicollis indicate the test for use in the immunodiagnosis of human neurocysticercosis.

【 授权许可】

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