Dot-enzyme-linked immunosorbent assay (Dot-ELISA) for detection of pneumococcal polysaccharide antigens in pleural fluid effusion samples.: Comparison with bacterial culture, counterimmunoelectrophoresis and latex agglutination

Revista do Instituto de Medicina Tropical de São Paulo

Henry I.z. Requejo²  Maria Das Graças A. Alkmin²  Regina G. Almeida¹  Silvana T. Casagrande¹  Ana Maria Cocozza¹  João Paulo B. Lotufo¹  Aurora R.p. Waetge¹  Joaquim C. Rodrigues¹ 

[1] ,Instituto Adolfo Lutz Seção de Imunologia São Paulo,Brasil

关键词: Dot-ELISA;    Pneumococcal antigen detection;    Streptococcus pneumoniae;    Pleural fluid effusion;    Bacterial pneumonia;   

DOI  :  10.1590/S0036-46651994000600010

来源: SciELO

【 摘 要 】

A dot-enzyme-linked immunosorbent assay (Dot-ELISA) for pneumococcal antigen detection was standardized in view of the need for a rapid and accurate immunodiagnosis of acute pneumococcal pneumonia. A total of 442 pleural fluid effusion samples (PFES) from children with clinical and laboratory diagnoses of acute bacterial pneumonia, plus 38 control PFES from tuberculosis patients and 20 negative control serum samples from healthy children were evaluated by Dot-ELISA. The samples were previously treated with 0.1 M EDTA pH 7.5 at 90°C for 10 min and dotted on nitrocellulose membrane. Pneumococcal omniserum diluted at 1:200 was employed in this assay for antigen detection. When compared with standard bacterial culture, counterimmunoelectrophoresis and latex agglutination techniques, the Dot-ELISA results showed relative indices of 0.940 to sensitivity, 0.830 to specificity and 0.760 to agreement. Pneumococcal omniserum proved to be an optimal polyvalent antiserum for the detection of pneumococcal antigen by Dot-ELISA. Dot-ELISA proved to be a practical alternative technique for the diagnosis of pneumococcal pneumonia.

【 授权许可】

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