Dot-ELISA-IgM in saliva for the diagnosis of human leptospirosis using polyester fabric-resin as support (Preliminary Report)

Revista do Instituto de Medicina Tropical de São Paulo

Marcos Vinicius Da Silva²  Paulo Mutuko Nakamura²  Eide Dias Camargo²  Luíza Batista¹  Adelaide José Vaz²  Angela Pires Brandão²  Antonio Walter Ferreira¹ 

[1] ,Instituto Adolfo LutzSao Paulo,Brasil

关键词: Leptospirosis;    Dot-ELISA;    Polyester-resin;    Solid-phase;    Saliva;   

DOI  :  10.1590/S0036-46651994000500014

来源: SciELO

【 摘 要 】

In order to improve the diagnosis of human leptospirosis, we standardized the dot-ELISA for the search of specific IgM antibodies in saliva. Saliva and serum samples were collected simultaneously from 20 patients with the icterohemorrhagic form of the disease, from 10 patients with other pathologies and from 5 negative controls. Leptospires of serovars icterohaemorrhagiae, canicola, hebdomadis, brasiliensis and cynopteri grown in EMJH medium and mixed together in equal volumes, were used as antigen at individual protein concentration of 0.2 µg/µl. In the solid phase of the test we used polyester fabric impregnated with N-methylolacrylamide resin. The antigen volume for each test was 1µl, the saliva volume was 8 µl, and the volume of peroxidase-labelled anti-human IgM conjugate was 30 µl. A visual reading was taken after development in freshly prepared chromogen solution. In contrast to the classic nitrocellulose membrane support, the fabric support is easy to obtain and to handle. Saliva can be collected directly onto the support, a fact that facilitates the method and reduces the expenses and risks related to blood processing.

【 授权许可】

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