Labeling of Salmonella Typhymurium with iodine-131 to study phagocytic function in rats

Revista do Instituto de Medicina Tropical de São Paulo

Maria K. Sato¹  Aldo J. Rodrigues Junior²  Edwaldo E. Camargo¹ 

[1] ,Universidade de São Paulo Faculdade de Medicina Departamento de RadiologiaSão Paulo SP ,Brasil

关键词: Salmonella typhymurium;    Iodine-131;    Transplanted spleen;   

DOI  :  10.1590/S0036-46651989000300001

来源: SciELO

【 摘 要 】

The present study describes a method for labeling Salmonella typhymurium with iodine-131 to evaluate both the morphological and the functional characteristics of the reticulo-endothelial system. A suspension containing 2 x 10(9) bacteria per ml was labeled with carrier-free Na131I without reductor, with a labeling yield of 46.5 ± 3% and 3.5 ± 1.3% of free Iodine-131. The biodistribution of the labeled bacteria in rats was studied with a large field-of-view scintillation camera equiped with a pinhole collimator. Whole body images were obtained 15 and 30 minutes after intravenous injection of the labeled microorganisms. Images showed accumulation of bacteria in the liver and both normal and transplanted spleens of the animals. Autoradiographs of liver and spleen demonstrated labeled bacteria within the cells of the reticulo-endothelial system. The method described is easy to perform, has a good labeling yield and allows the functional evaluation of the reticulo-monophagocytic system, including transplanted spleens.

【 授权许可】

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