Evaluation of genotoxicity induced by repetitive administration of local anaesthetics: an experimental study in rats

Revista Brasileira de Anestesiologia

Gisele Alborghetti Nai¹  Mariliza Casanova De Oliveira¹  Graziela De Oliveira Tavares¹  Laís Fabrício Fonseca Pereira¹  Nádia Derli Salvador Lemes Soares¹  Patrícia Gatti Silva¹ 

关键词: Anaesthesia;    Genotoxicity;    Mutagenicity tests;    Micronucleus tests;    Prilocaine;    Anestesia;    Genotoxicidade;    Testes de mutagenicidade;    Testes para micronúcleos;    Prilocaína;    Anestesia;    Genotoxicidad;    Tests de mutagenicidad;    Tests de micronúcleos;    Prilocaína;   

DOI  :  10.1016/j.bjane.2013.07.006

来源: SciELO

【 摘 要 】

BACKGROUND AND OBJECTIVE: Previous studies regarding the effects of some local anaesthetics have suggested that these agents can cause genetic damage. However, they have not been tested for genotoxicity related to repetitive administration. The aim of this study was to evaluate the genotoxic potential of local anaesthetics upon repetitive administration. METHODS: 80 male Wistar rats were divided into: group A - 16 rats intraperitoneally injected with lidocaine hydrochloride 2%; group B - 16 rats IP injected with mepivacaine 2%; group C - 16 rats intraperitoneally injected with articaine 4%; group D - 16 rats IP injected with prilocaine 3% (6.0 mg/kg); group E - 8 rats subcutaneously injected with a single dose of cyclophosphamide; and group F - 8 rats intraperitoneally injected with saline. Eight rats from groups A to D received a single dose of anaesthetic on Day 1 of the experiment; the remaining rats were dosed once a day for 5 days. RESULTS: The median number of micronuclei in the local anaesthetics groups exposed for 1 or 5 days ranged from 0.00 to 1.00, in the cyclophosphamide-exposed group was 10.00, and the negative control group for 1 and 5 days was 1.00 and 0.00, respectively (p < 0.0001). A significant difference in the number of micronuclei was observed between the cyclophosphamide group and all local anaesthetic groups (p = 0.0001), but not between the negative control group and the local anaesthetic groups (p > 0.05). CONCLUSION: No genotoxicity effect was observed upon repetitive exposure to any of the local anaesthetics evaluated.

【 授权许可】

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