Cerebrospinal fluid can be used for HIV genotyping when it fails in blood

Arquivos de Neuro-Psiquiatria

Indianara Rotta¹  Sonia Mara Raboni¹  Cléa Elisa Lopes Ribeiro¹  Maristela Riedel¹  Maria Da Graça Winhescki¹  Davey M. Smith¹  Ronald J. Ellis¹  Sérgio Monteiro De Almeida¹ 

关键词: HIV-1;    clades;    genotyping;    cerebrospinal fluid;    central nervous system;    HIV-1;    subtipos;    genotipagem;    líquido cefalorraquidiano;    sistema nervoso central;   

DOI  :  10.1590/0004-282X20140093

来源: SciELO

【 摘 要 】

Blood plasma specimens are the clinical standard for HIV-1 pol gene genotyping from viral populations; however, it is not always successful, often from low viral loads or the presence of polymerase chain reaction (PCR) inhibitors.Objective To describe the successful of HIV-1 genotyping in two samples of cerebrospinal fluid (CSF), after genotype procedures failed from blood. Method Two HIV-infected patients enrolled in a neurocognitive research study were evaluated when standard HIV-1 genotyping failed from blood plasma samples. Genotyping was performed using the commercial system TRUGENE® HIV-1 Genotyping Kit and the OpenGene® DNA Sequencing System (Siemens Healthcare Diagnostics, Tarrytown, NY, USA). Results CSF genotyping was performed via the same commercial platform and was successful in both cases. Conclusion This report demonstrates that CSF could be used as an alternate clinical specimen for HIV-1 genotyping when it fails from blood.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License