Transcription analysis of TIMP-1 and NM23-h1 genes in glioma cell invasion

Arquivos de Neuro-Psiquiatria

José Augusto Nasser²  Asdrúbal Falavigna¹  Fernando Ferraz²  Gregory Duigou¹  Jeffrey Bruce¹ 

[1] ,Universidade Federal de São Paulo Escola Paulista de MedicinaSP ,Brazil

关键词: tumor cell invasion;    TIMP-1;    NM23-H1;    RNA levels;    gliomas;    invasão celular tumoral;    TIMP-1;    NM23-H1;    níveis de RNA;    gliomas;   

DOI  :  10.1590/S0004-282X2006000500014

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate using transcription analysis the presence and importance of two genes: NM23-H1 and TIMP-1 on control of tumor cell invasion in diffuse astrocytomas (WHO II) and glioblastoma multiforme (WHO IV). METHOD: Northern blot analysis of NM23-H1 and TIMP-1 was performed. Eight diffuse astrocytomas and 19 glioblastomas (WHO IV) were analyzed to determine if TIMP-1 and NM23-H1 were candidates to inhibition of tumor cell invasion quantitated RNA levels. The samples were collected directly from operating room. Total cellular RNA was extracted from frozen tissue samples using guanidinium-isothiocyanate and cesium chloride gradients. Total RNA (10 mg per sample) from tumor tissue were size fractionated through 1% agarose-formaldehyde gel and transferred to nylon filters and then hybridized to 32P-labeled DNA probes and placed for autoradiography. Levels of specific RNAs were determined by computer-assisted laser densitometry. Blot filters were sequentially hybridized to nm23 and TIMP-1 probes in addition to GAPDH, as a control. Statistical analyses were carried out according to t-test for equality of means. RESULTS: NM23-H1 were detected in each sample, however it did not correlate with malignancy and invasiveness. On the other side TIMP-1 gene expression showed a clear correlation between low expression and invasiveness. CONCLUSION: The data suggest that TIMP-1 is an inhibitor of high grade gliomas invasion. NM23-H1 was present in the entire gliomas sample, but it did not vary in diffuse astrocytomas and glioblastomas.

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