Regional differences in the number and type of myenteric neurons in the descending colon of rats

Arquivos de Neuro-Psiquiatria

Eduardo José De Almeida Araújo²  Débora De Mello Gonçales Sant'ana²  Sônia Lucy Molinari¹  Marcílio Hubner De Miranda Neto¹ 

[1] ,Universidade ParanaenseUmuarama PR ,Brasil

关键词: myenteric plexus;    Giemsa;    NADH-diaphorase;    NADPH-diaphorase;    rat;    descending colon;    plexo mioentérico;    Giemsa;    NADH-diaforase;    NADPH-diaforase;    rato;    colo descendente;   

DOI  :  10.1590/S0004-282X2003000200011

来源: SciELO

【 摘 要 】

The purpose of this study was to analyze the neuronal density of the myenteric plexus of the intermediate and antimesocolic regions of the descending colon of rats. Whole-mounts were stained with three different techniques of neuronal evidenciation. Through counts of the number of neurons in an area of 6.64 mm² under light microscopy, we found 1,271 ± 227.54 neurons with Giemsa in the intermediate region and 1,234 ± 225.92 neurons in the antimesocolic region; with the NADH-diaphorase technique we found 530 ± 92.97 neurons in the intermediate region and 539 ± 146.72 neurons in the antimesocolic region; and through the NADPH-diaphorase histochemistry, we found 417 ± 34.42 neurons in the intermediate region and 547 ± 84.01 neurons in the antimesocolic region. We conclude that there is a variation in the density of NADPH-diaphorase positive neurons in the intestinal circumference; that the NADH-diaphorase positive neuronal subpopulation represented 42.7% of that stained with Giemsa; and that the NADPH-diaphorase positive neurons represented 37.8% of the whole myenteric population.

【 授权许可】

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