Detection of Toxoplasma gondii DNA in peripheral blood and aqueous humor of patients with Toxoplasmic active focal necrotizing retinochoroiditis using real-time PCR

Arquivos Brasileiros de Oftalmologia

Fabio Felipe Dos Santos¹  Heloisa Nascimento¹  Cristina Muccioli¹  Deise Fialho Da Costa¹  Luiz Vicente Rizzo¹  Alessandra Gonçalves Commodaro¹  Rubens Belfort Jr.¹ 

关键词: Toxoplasmosis;    ocular/diagnosis;    Toxoplasma;    Blood/parasitology;    Choroiditis;    Real-time polymerase techniques/methods;    Aqueous humor;    Toxoplasmose ocular/diagnóstico;    Toxoplasma;    Sangue/parasitologia;    Coriodite;    Reação em cadeia da polimerase em tempo real;    Humor aquoso;   

DOI  :  10.5935/0004-2749.20150094

来源: SciELO

【 摘 要 】

ABSTRACTPurpose: To evaluate the ability of real-time quantitative PCR (qPCR) for detectingToxoplasma gondii DNA in the peripheral blood and aqueous humor of patients with toxoplasmic active focal necrotizing retinochoroiditis.Methods: Fifty-five patients with infectious uveitis seen from 2009 to 2013 at the Department of Ophthalmology and Visual Sciences of the Federal University of São Paulo were enrolled in this study. Forty-three patients had toxoplasmic active focal necrotizing retinochoroiditis, and the remaining 12 had non-toxoplasmic infectious uveitis and served as controls. qPCR analysis forT. gondii DNA was performed on the patients' peripheral blood and aqueous humor samples.Results: The qPCR was positive for T. gondii DNA in 37.21% (16/43) of the aqueous humor samples and 2.33% (1/43) of the peripheral blood samples; further, 16.27% (7/43) of the patients had positive results in both their blood and aqueous humor samples.Conclusion: qPCR was able to detect T. gondii DNA in patients with toxoplasmic active focal necrotizing retinochoroiditis in the blood as well as the aqueous humor and can help with the diagnosis of the disease.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License