Effect of topical 0.5% povidone-iodine compared to 5% natamycin in fungal keratitis caused by Fusarium solani in a rabbit model: a pilot study

Arquivos Brasileiros de Oftalmologia

Lauro Augusto De Oliveira¹  Thales Takeo Takata¹  Alvio Isao Shiguematsu¹  Luiz Alberto Soares Melo Júnior¹  Olga Fischman Gompertz¹  Luciene Barbosa De Sousa¹  Mark J. Mannis² 

[1] ,Universidade Federal de São PauloSão Paulo SP ,Brazil

关键词: Keratitis;    Povidone-iodine;    Povidone-iodine;    Fusarium;    Natamycin;    Natamycin;    Eye infections;    fungal;    Antifungal agents;    Antifungal agents;    Ceratite;    Povidona-Iodo;    Povidona-Iodo;    Fusarium;    Natamicina;    Natamicina;    Infecções oculares fúngicas;    Antimicóticos;    Antimicóticos;   

DOI  :  10.1590/S0004-27492008000600018

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate the efficacy of topical administration of 0.5% povidone-iodine in experimental Fusarium solani keratitis in rabbits. METHODS: Fungal keratitis caused by Fusarium solani was induced in the right eye of 24 New Zealand rabbits. The rabbits were randomly divided into 3 different treatment groups: Group I (povidone-iodine) - treated with topical 0.5% povidone-iodine; Group II (natamycin) - treated with topical 5% natamycin; and Group III (control) - treated with topical saline solution. In all groups the rabbits were treated for three days and then sacrificed. The corneas were excised, macerated and immersed in 10 mL BHI. Culture samples were plated daily on Sabouraud's agar for 7 days, and the number of colony-forming units (CFU) was counted. The rabbits were clinically evaluated during the treatment period. RESULTS: The povidone-iodine and natamycin groups demonstrated better efficacy than the control group based on the number of rabbits with no colonies growing. However, there were no statistically significant differences between the three groups when the number of CFU was analyzed (p>0.05). CONCLUSIONS: Our study demonstrates important methodological considerations in the use of in vivo animal models for the testing of antifungal agents. Using this sample size and methodology of counting CFU, topical 0.5% povidone-iodine demonstrated no benefit in the treatment of experimental Fusarium solani when compared with topical 5% natamycin.

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