Ameliorative potential of Ocimum sanctum in chronic constriction injury-induced neuropathic pain in rats

Anais da Academia Brasileira de Ciências

Gurpreet Kaur¹  Anjana Bali¹  Nirmal Singh¹  Amteshwar S. Jaggi¹ 

关键词: chronic constriction injury;    Ocimum sanctum;    Saponin rich fraction;    calcium;    oxidative stress;    lesão por constrição crônica;    Ocimum sanctum;    saponinas;    cálcio;    estresse oxidativo;   

DOI  :  10.1590/0001-3765201520130008

来源: SciELO

【 摘 要 】

The present study was designed to investigate the ameliorative potential of Ocimumsanctum and its saponin rich fraction in chronic constriction injury-induced neuropathic pain in rats. The chronic constriction injury was induced by placing four loose ligatures around the sciatic nerve, proximal to its trifurcation. The mechanical hyperalgesia, cold allodynia, paw heat hyperalgesia and cold tail hyperalgesia were assessed by performing the pinprick, acetone, hot plate and cold tail immersion tests, respectively. Biochemically, the tissue thio-barbituric acid reactive species, super-oxide anion content (markers of oxidative stress) and total calcium levels were measured. Chronic constriction injury was associated with the development of mechanical hyperalgesia, cold allodynia, heat and cold hyperalgesia along with an increase in oxidative stress and calcium levels. However, administration of Ocimumsanctum (100 and 200 mg/kg p.o.) and its saponin rich fraction (100 and 200 mg/kg p.o.) for 14 days significantly attenuated chronic constriction injury-induced neuropathic pain as well as decrease the oxidative stress and calcium levels. It may be concluded that saponin rich fraction of Ocimum sanctum has ameliorative potential in attenuating painful neuropathic state, which may be attributed to a decrease in oxidative stress and calcium levels.

【 授权许可】

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