Anais da Academia Brasileira de Ciências | |
Natural sesquiterpen lactones as acetylcholinesterase inhibitors | |
Homa Hajimehdipoor1  Mahmoud Mosaddegh1  Farzaneh Naghibi1  Ali Haeri1  Maryam Hamzeloo-moghadam1  | |
关键词: Acetylcholinesterase inhibitor; Alzheimer; britannin; gaillardin; Inula; pulchellin C; inibidor de acetilcolinesterase; Alzheimer; britanina; gailardina; Inula; pulchelina C; | |
DOI : 10.1590/0001-3765201420130005 | |
来源: SciELO | |
![]() |
【 摘 要 】
Background and the purpose of the study: The amount of elder people who suffer from Alzheimer disease is continuously increasing every year. Cholinesterase inhibitors have shown to be effective in alleviating the symptoms of the disease, thus opening a field of research for these treatments. Herbal products, owning a reputation as effective agents in many biological studies are now drawing attention for inhibiting acetylcholinesterase, in other words, Alzheimer disease. In the present study, the ability of three sesquiterpene lactones from Inula oculus-christi and I. aucheriana to inhibit AChE has been evaluated through Ellman assay.Materials and Methods: Gaillardin and pulchellin C were obtained from I. oculus-christi and britannin from I. aucheriana by chromatographic methods. They were dissolved in methanol in concentration of 3 mg/mL and the AChEI activity of the compounds was determined by Ellman method using Acethylthiocholine iodide as the substrate and 5, 5′-dithiobis-2-nitrobenzoic acid as the reagent, in 96-well plates at 405 nm.Results: AChEI activity of the examined compounds was obtained as 67.0, 25.2 and 10.9% in concentration of 300 µg/L for gaillardin, britannin and pulchellin C, respectively.Conclusion: Among the three sesquiterpene lactones, gaillardin with 67% inhibition of AChE could be considered a good candidate for future Alzheimer studies.
【 授权许可】
CC BY
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202103040001264ZK.pdf | 446KB | ![]() |