Cryopreservation of mutton snapper (Lutjanus analis) sperm

Anais da Academia Brasileira de Ciências

Eduardo G. Sanches¹  Idili R. Oliveira¹  Pedro C. Da Silva Serralheiro¹  Vinicius R. Cerqueira¹ 

关键词: cryoprotector;    extender;    Lutjanus analis;    reproduction;    sperm;    crioprotetor;    diluidores;    Lutjanus analis;    reprodução;    sêmen;   

DOI  :  10.1590/S0001-37652013005000047

来源: SciELO

【 摘 要 】

This study aimed to develop a protocol of semen cryopreservation of the mutton snapper Lutjanus analis. The interaction between three extenders ( pH 6.1; 7.8 and 8.2) , two concentrations of dimethyl sulfoxide ( DMSO, 5 and 10%) and three cooling rates ( -90; -60 and -30°C.min−1) on the sperm motility rate and motility time were analyzed by a factorial experiment. A sample of 30 fishes ( 1,261 ± 449 g) collected in the nature was kept in floating net cages. The semen was frozen by using cryogenic straws, in nitrogen vapour and transferred, later, to liquid nitrogen. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The highest sperm motility rate and motility time ( P < 0.05) was achieved by combining extender C ( pH 8.2) with DMSO ( 10%) and cooling rate of -60°C.min−1 ( P < 0.05) . The use of cryopreserved sperm presented fertilization rates higher than 59% validating the present protocol for mutton snapper.

【 授权许可】

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