Study of the antiproliferative potential of seed extracts from Northeastern Brazilian plants

Anais da Academia Brasileira de Ciências

Paulo Michel P. Ferreira²  Davi F. Farias¹  Martônio P. Viana¹  Terezinha M. Souza¹  Ilka M. Vasconcelos¹  Bruno M. Soares¹  Cláudia Pessoa¹  Letícia V. Costa-lotufo¹  Manoel O. Moraes¹  Ana F.u. Carvalho¹ 

[1] ,Universidade Federal do Piauí Departamento de Ciências Biológicas Picos PI ,Brasil

关键词: antiproliferative potential;    Northeastern Brazilian plants;    Myracrodruon urundeuva;    sarcoa 180 tumor;    seed extracts;    potencial antiproliferativo;    plantas do Nordeste Brasileiro;    Myracrodruon urundeuva;    tumor sarcoma 180;    Extratos de sementes;   

DOI  :  10.1590/S0001-37652011005000017

来源: SciELO

【 摘 要 】

This study assessed the antiproliferative and cytotoxic potential against tumor lines of ethanolic seed extracts of 21 plant species belonging to different families from Northeastern Brazil. In addition, some underlying mechanisms involved in this cytotoxicity were also investigated. Among the 21 extracts tested, the MTT assay after 72 h of incubation demonstrated that only the ethanolic extract obtained from Myracrodruon urundeuva seeds (EEMUS), which has steroids, alkaloids and phenols, showed in vitro cytotoxic activity against human cancer cells, being 2-fold more active on leukemia HL-60 line [IC50 value of 12.5 (9.5-16.7) μg/mL] than on glioblastoma SF-295 [IC50 of 25.1 (17.3-36.3) μg/mL] and Sarcoma 180 cells [IC50 of 38.1 (33.5-43.4) μg/mL]. After 72h exposure, flow cytometric and morphological analyses of HL-60-treated cells showed that EEMUS caused decrease in cell number, volume and viability as well as internucleosomal DNA fragmentation in a dose-dependent way, suggesting that the EEMUS triggers apoptotic pathways of cell death.

【 授权许可】

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